Additional file 2.
Western blot analysis of CalpA expression in different developmental stages ofDrosophila. Extracts prepared from early (stage 2) and late (stage 17) embryos ( A), 1st ,2nd and 3rd instar larvae, pupae, as well as from adult male and female flies (B), were separated by SDS-PAGE and probed with anti-CalpA antibody. 20 ng recombinant CalpA was loaded in the control lane. The positions of the molecular mass standards are denoted. Arrows indicate the main protein bands stained with the CalpA-specific antibody. The positions of internal standards α-tubulin in panel A and α-actin in panel B are also indicated by arrows.
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Kókai et al. BMC Developmental Biology 2012 12:20 doi:10.1186/1471-213X-12-20