The development of bone and cartilage in the tammar limb. Alcian blue staining of (A) day 23 fetus and (D) day 25 fetus. A diagrammatic representation of alcian blue stained cartilage elements in a day 23 fetus (B) forelimb, (C) hindlimb and day 25 fetus (E) forelimb and (F) hindlimb. Staining show the early skeletal element deposited as cartilage was clearly visible in the forelimb before birth but not in the hindlimb. By day 25 of pregnancy, the forelimb was well formed and the hindlimb shows early cartilage elements. Digits are numbered with Roman numerals.
Chew et al. BMC Developmental Biology 2012 12:2 doi:10.1186/1471-213X-12-2