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Open Access Research article

HOXA13 and HOXD13 expression during development of the syndactylous digits in the marsupial Macropus eugenii

Keng Yih Chew12, Hongshi Yu12, Andrew J Pask123, Geoffrey Shaw12 and Marilyn B Renfree12*

Author Affiliations

1 ARC Centre of Excellence in Kangaroo Genomics, The University of Melbourne, Melbourne, Victoria, 3010, Australia

2 Department of Zoology, The University of Melbourne, Melbourne, Victoria, 3010, Australia

3 Department of Molecular and Cellular Biology, The University of Connecticut, Storrs, CT 06269, USA

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BMC Developmental Biology 2012, 12:2  doi:10.1186/1471-213X-12-2

Published: 11 January 2012

Additional files

Additional file 1:

Table S1: Protein sequences of HOXA13 and HOXD13. The sequences used in this study were retrieved from GenBank or Ensembl.

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Additional file 2:

Phylogenetic tree of HOXA13 and HOXD13. The evolutionary history was inferred using the Neighbor-Joining method [56]. The bootstrap consensus tree inferred from 1000 replicates is taken to represent the evolutionary history of the taxa analyzed [57]. Branches corresponding to partitions reproduced in less than 50% bootstrap replicates were collapsed. The percentage of replicate trees in which the associated taxa clustered together in the bootstrap test (1000 replicates) are shown next to the branches [57]. The evolutionary distances were computed using the Poisson correction method [37] and are in the units of the number of amino acid substitutions per site. The analysis involved 24 amino acid sequences with a total of 114 positions in the final dataset. All positions containing gaps and missing data were eliminated. Evolutionary analyses were conducted in MEGA5 [37].

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