Additional file 9.

Immunohistochemistry of DHH, PTCH1 and PTCH2 in the tammar wallaby testis at key developmental time points. Red/brown staining indicates protein distribution while the heamatoxalin counterstain appears blue. It is important to note that DHH is a highly secreted molecule and staining does not imply cell of origin. DHH was initially present at high levels throughout the indifferent gonad (d24 fetus), by D1pp Dhh is confined to the aggregating seminiferous cords (AC). Scale bars = 36 μm.

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O'Hara et al. BMC Developmental Biology 2011 11:72   doi:10.1186/1471-213X-11-72