Additional file 10.

Immunohistochemistry of DHH, PTCH1 and PTCH2 in the tammar wallaby ovary at key developmental time points. At day 9pp when the ovary is forming a cortex and medulla, there was widespread staining for DHH, PTCH1 and PTCH2 throughout the ovary. By D72pp DHH and PTCH1 were concentrated in the germ cell nests (CGN) and PTCH2 was largely in the interstitium. In the adult ovary, DHH was found in the granulosa cells (GC) of follicles at all stages of development, and in the oocyte cytoplasm. Staining was also observed in the corpus luteum (CL). Scale bars = 40 μm at D9, D72 and 160 μm in the corpus luteum.

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O'Hara et al. BMC Developmental Biology 2011 11:72   doi:10.1186/1471-213X-11-72