Additional file 5.

Immunohistochemistry for EGFP driven by pax9 enhancers. (A) Enhancer Px2 driven EGFP expresses in the sclerotomal cells (black arrow) at 36 hpf. The red arrow indicates background staining. Similar expression domains can be seen for enhancer Px4 (B) at 36 hpf and Px7 (C) at 48 hpf. (D) the negative control (empty vector injection) shows no staining in the sclerotome. (E) Section RNA in situ hybridization for pax9 at 36 hpf.

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Chatterjee et al. BMC Developmental Biology 2011 11:63   doi:10.1186/1471-213X-11-63