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Open Access Highly Accessed Research article

Identification of RNA binding motif proteins essential for cardiovascular development

Samantha Maragh12, Ronald A Miller2, Seneca L Bessling2, David M McGaughey2, Marja W Wessels3, Bianca de Graaf3, Eric A Stone4, Aida M Bertoli-Avella3, John D Gearhart5, Shannon Fisher6 and Andrew S McCallion17*

Author Affiliations

1 Biochemical Science Division, National Institute of Standards and Technology, Gaithersburg, MD 20899, USA

2 McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University School of Medicine, Baltimore, MD, 21205, USA

3 CBG Department of Clinical Genetics, Erasmus Medical Centre, 3016 AH Rotterdam, The Netherlands

4 Department of Genetics and Bioinformatics Research Center, North Carolina State University, Raleigh, NC, 27695-7566, USA

5 Institute for Regenerative Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA

6 Department of Cell and Developmental Biology, University of Pennsylvania, Philadelphia, PA 19104, USA

7 Department of Molecular and Comparative Pathobiology, Johns Hopkins University School of Medicine, Baltimore MD, 21205, USA

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BMC Developmental Biology 2011, 11:62  doi:10.1186/1471-213X-11-62

Published: 19 October 2011



We recently identified Rbm24 as a novel gene expressed during mouse cardiac development. Due to its tightly restricted and persistent expression from formation of the cardiac crescent onwards and later in forming vasculature we posited it to be a key player in cardiogenesis with additional roles in vasculogenesis and angiogenesis.


To determine the role of this gene in cardiac development, we have identified its zebrafish orthologs (rbm24a and rbm24b), and functionally evaluated them during zebrafish embryogenesis. Consistent with our underlying hypothesis, reduction in expression of either ortholog through injection of morpholino antisense oligonucleotides results in cardiogenic defects including cardiac looping and reduced circulation, leading to increasing pericardial edema over time. Additionally, morphant embryos for either ortholog display incompletely overlapping defects in the forming vasculature of the dorsal aorta (DA), posterior caudal vein (PCV) and caudal vein (CV) which are the first blood vessels to form in the embryo. Vasculogenesis and early angiogenesis in the trunk were similarly compromised in rbm24 morphant embryos at 48 hours post fertilization (hpf). Subsequent vascular maintenance was impaired in both rbm24 morphants with substantial vessel degradation noted at 72 hpf.


Taken collectively, our functional data support the hypothesis that rbm24a and rbm24b are key developmental cardiac genes with unequal roles in cardiovascular formation.