Protein expression in the "remodeling bile duct" stage at 15½ weeks post conception. (A). Graphical interpretation of the "remodeling bile duct" stage visualizing the formation of bile ducts from the ductal plate and their remodeling into the portal mesenchyma. (B-E) At this time in development CK7 (b, arrow) was expressed in the ductal plates and bile ducts, while the hepatocytes expressed HepPar1 (E) and were negative for CK19 (C) and EpCAM (D). Some bile ducts were only weakly positive for EpCAM (d, arrow). Expression of SOX9 (F), laminin (G) and nestin (H) was unaltered from 9½ weeks post conception. AQP1 (I) stained the biliary structures (arrows). Upper case letters x10, lower case letters x63. Scale bar: 100 μm (for all photos marked with upper cases).
Vestentoft et al. BMC Developmental Biology 2011 11:56 doi:10.1186/1471-213X-11-56