Cug2 is essential for normal mitotic control and CNS development in zebrafish
1 Department of Biology, Chungnam National University, Daejeon, South Korea
2 Department of Microbiology, Chungnam National University, Daejeon, South Korea
3 Genome Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, 305-764, South-Korea
4 Biomedical Sciences, St. George's Medical School, University of London, London, SW17 0RE, UK
BMC Developmental Biology 2011, 11:49 doi:10.1186/1471-213X-11-49Published: 15 August 2011
We recently identified a novel oncogene, Cancer-upregulated gene 2 (CUG2), which is essential for kinetochore formation and promotes tumorigenesis in mammalian cells. However, the in vivo function of CUG2 has not been studied in animal models.
To study the function of CUG2 in vivo, we isolated a zebrafish homologue that is expressed specifically in the proliferating cells of the central nervous system (CNS). Morpholino-mediated knockdown of cug2 resulted in apoptosis throughout the CNS and the development of neurodegenerative phenotypes. In addition, cug2-deficient embryos contained mitotically arrested cells displaying abnormal spindle formation and chromosome misalignment in the neural plate.
Therefore, our findings suggest that Cug2 is required for normal mitosis during early neurogenesis and has functions in neuronal cell maintenance, thus demonstrating that the cug2 deficient embryos may provide a model system for human neurodegenerative disorders.