Multi-species conserved sequences at the ERBB3 drive reporter activity in neural crest derived lines and exhibit epigenetic marks consistent with enhancer activity. (A) Schematic representation of the eight multi-species conserved sequences at the human ERBB3 locus. (B) Luciferase activity of ERBB3_MCS1-8 in melan-a cells. (C) Luciferase activity of ERBB3_MCS1-8 in S16 cells. (D) Luciferase activity of ERBB3_MCS1-8 in NIH3T3 cells. All luciferase values are normalized to internal renilla control and are shown as fold-change in reporter activity as compared to the promoter only construct (pe1b) with standard deviation. (E) Real-time PCR results of Chromatin Immunoprecipitation. Black bar indicates enrichment upon H3K4me1 ChIP whereas grey bar indicates enrichment with non-specific IgG. Error bars indicate standard deviation of technical replicates in real-time PCR. Elements MCS1-8 represent the mouse orthologs of ERBB3_MCS elements. C refers to a region 3 Mb upstream of Erbb3, which was used as a negative control.
Prasad et al. BMC Developmental Biology 2011 11:40 doi:10.1186/1471-213X-11-40