Table 2 |
|
|
Primer sequences |
|
|
Primer name |
Sequence |
|
|
|
|
human RT-PCR |
TTAGTGCATGGTCAGGTGAGG |
|
mouse RT-PCR |
TCTCCCATCTTCACTCTCATCAG |
|
chicken RT-PCR |
GCTGTGTTCTGACTTCATCC |
|
hten PCR1 rev |
GTGTCCACATCAGATCCCATCTC |
|
hten nested XbaI rev |
TAGTTCTAGAGCACAGGTGCAGGCATGAGG |
|
mouse PCR1 rev |
CTCCAGCTGGTAGCCATGTCG |
|
mouse nested XbaI rev |
TAGTTCTAGATCTGTGTGGTAGCCGGAGCAC |
|
chicken PCR1 rev |
ATGCGCCACCTTCGGTTTCAG |
|
chicken nested XbaI rev |
TAGTTCTAGAGTAGCTGTGCTCCGACACACC |
|
oligo dT anchor primer |
GACCACGCGTATCGATGTCGACTTTTTTTTTTTTTTTT |
|
hten1 promotor 1 XhoI fw |
ACTACTCGAGCAAGACCCATGCTGAAGCT |
|
hten1 promotor 1 HindIII rev |
ACTAAAGCTTCTCTGATTAAGCAAGCTCAGTTTC |
|
hten1 promoter 2 NheI fw |
ACTAGCTAGCCCCCTAGAGTGTTCAGCTCT |
|
hten1 promoter 2 EcoRI rev |
ACTAGAATTCGGGCCACCTCAAAAACACCTCC |
|
mutate promoter 2 fw |
CCACCCCTCACCCTCAGGAGAAAGTGGTTAAA |
|
mutate promoter 2 rev |
TTTAACCACTTTCTCCTGAGGGTGAGGGGTGG |
|
total ten1 qPCR fw |
GCATAGTTCCTGTTTGTCCA |
|
total ten1 qPCR rev |
TCTGCACATCTTGAGTAGAC |
|
alt exon qPCR fw |
GCTTGGAATAGGAATTACAAGG |
|
alt exon qPCR rev |
GAAGTCCTTTAATGCAAGCAG |
|
hGAPDH qPCR fw |
GGAGTCAACGGATTTGGTC |
|
hGAPDH qPCR rev |
AAACCATGTAGTTGAGGTC |
|
ChIP target region fw |
TTCAGCTTCCTCGTTCTTCG |
|
ChIP target region rev |
GGTGGTTACAACCGCCTTTT |
|
ChIP negative control fw |
AGATTCCTGTGAGCCCTGCT |
|
ChIP negative control rev |
TCCAACAACTCATGCAATGG |
|
|
|
|
Beckmann et al. BMC Developmental Biology 2011 11:35 doi:10.1186/1471-213X-11-35 |
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