Additional file 4.

Sham surgery triggers a cell-cycle entry of the cardiomyocytes in the vicinity the epicardium. (A-B) The nuclei of cardiomyocytes express DsRed2-Nuc protein under the control of cmlc-2 promoter. Tropomyosin (blue) labels the myocardium, MCM5 (green) is expressed in the mitotic cells, DAPI marks all the nuclei. Proliferating cardiomyocytes are identified by the co-expression of DsRed2-Nuc and MCM5 (circles). (A' and B') Higher magnifications of framed area shown in left panels. (A-A') Uninjured animals display a very few proliferating cardiomyocytes in the ventricle. (B-B') The ventricle of animals at 4 days sham-operation contains an enhanced number of DsRed2-Nuc/MCM5-positive nuclei at the myocardial periphery, underneath the epicardium. Bars in (A-A') represent 300 μm.

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Chablais et al. BMC Developmental Biology 2011 11:21   doi:10.1186/1471-213X-11-21