Expression of tra-2 of S. ocellaris and B. coprophila. (A) Molecular organisation (only exons are shown) of the Sciara tra-2 transcript with the primers used. The meaning of the colours is explained in the legend to Figure 1. (B) RT-PCR analyses of total RNA from S. ocellaris embryos (E), larvae (L), whole male adults (M), testis (T), adult male abdomens (MA), adult male head plus thorax (MHT), whole adult females (F), ovaries (O), adult female abdomens (MA), and adult female head plus thorax (MHT). (C) RT-PCR analyses of total RNA from B. coprophila whole male adults (M), male embryos (ME), male larvae (ML), adult male head plus thorax (MHT), testis (T), whole female adults (F), female embryos (FE), female larvae (FL), adult female head plus thorax (FHT) and ovaries (O). Protein L10 was used as a control of the expression of the gene rpL10, which codes for the constitutive ribosomal protein.
Martín et al. BMC Developmental Biology 2011 11:19 doi:10.1186/1471-213X-11-19