Table 3

The developmental potential of mouse embryos cultured in vitro after microinjection of different concentrations of granzyme G antisense morpholinos into the cytoplasm of one-cell stage fertilized eggs

Dosage of Granzyme G Morpholino

Total embryo treated in one-cell stage

No. (%) of survival embryo after treatment

No. (%) of embryos developed to


2-cell embryo

4-cell embryo

8-cell embryo

Morula embryo

Blastocyst embryo


2 mM

64

31(48.4)

25(80.7)a

7(22.6)a

0(0.0)a

0(0.0)a

0(0.0)a

0.2 mM

65

33(50.8)

32(97.0) b

29(87.9)b

25(75.8)b

24(72.7)b

11(33.3)b

0.02 mM

81

45(55.6)

45(100.0)b

45(100.0)c

43(95.6)c

43(95.6)c

43(95.6)c

0.002 mM

63

39(61.9)

38(97.4)b

36(92.3)bc

34(87.2)bc

34(87.2)bc

34(87.2)c


Six experiments were carried out in each group, and 10-15 embryos were used in each experiment.

abc Different superscripts within a column indicate a statistically significant difference (p < 0.05).

Tsai et al. BMC Developmental Biology 2010 10:88   doi:10.1186/1471-213X-10-88

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