Table 2

The effects of microinjection of different substrates into the cytoplasm of one-cell stage fertilized eggs on early embryo developmental potential

Different substrate treatment

Total embryo treated in one-cell stage

No.(%) of survival embryo after treatment

No.(%) of embryos developed to


2-cell embryo

4-cell embryo

8-cell embryo

Morula embryo

Blastocyst embryo


2 mM granzyme G Morpholino

80

43(53.8)

39(90.7)a

5(11.6)a

0(0.0)a

0(0.0)a

0(0.0)a

2 mM control Morpholino

90

45(50.0)

45(100.0)ab

42(93.3)b

42(93.3)b

37(82.2)b

26(57.8)b

M2 buffer injection

46

20(43.5)

20(100.0)ab

17(85.0)b

17(85.0)bc

17(85.0)b

17(85.0)c

Control embryo

(Uninjected)

74

--

74(100.0)b

70(94.6)b

57(77.0)c

57(77.0)b

57(77.0)bc


Six experiments were carried out in each group, and 10-15 embryos were used in each experiment.

abc Different superscripts within a column indicate a statistically significant difference (p < 0.05).

Tsai et al. BMC Developmental Biology 2010 10:88   doi:10.1186/1471-213X-10-88

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