Quantitation of zygotic RNA synthesis during the maternal-zygotic transition at the two-cell stage of the mouse embryo. (A to D) Phase contrast images. (E to H) Images of a two-cell embryo treated with BrUTP and stained with Cy5-conjugated anti-BrdUTP antibody (red). (I to L) mRNA expression profiles of the green lines presented in panels E, F, G, and H, respectively. (A, E, I) Two-cell stage embryo following microinjection of granzyme G morpholino. (B, F, J) Two-cell stage embryo after injection of control morpholino. (C, G, K) Two-cell stage of a normal embryo. (D, H, L) Two-cell stage embryo pretreated with α-amanitin, a transcriptional inhibitor, as a negative control. Scale bar: 25 μm.
Tsai et al. BMC Developmental Biology 2010 10:88 doi:10.1186/1471-213X-10-88