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Open Access Highly Accessed Research article

Emergence and migration of trunk neural crest cells in a snake, the California Kingsnake (Lampropeltis getula californiae)

Michelle Reyes1, Katrina Zandberg1, Iska Desmawati2 and Maria E de Bellard1*

Author Affiliations

1 California State University Northridge, Biology Dept., MC 8303. 18111 Nordhoff Street., Northridge, CA 91330, USA

2 Institute Technology Sepuluh Nopember, Biology Department, Faculty of Mathematics and Natural Science, Surabaya, Indonesia

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BMC Developmental Biology 2010, 10:52  doi:10.1186/1471-213X-10-52

Published: 18 May 2010

Abstract

Background

The neural crest is a group of multipotent cells that emerges after an epithelial-to-mesenchymal transition from the dorsal neural tube early during development. These cells then migrate throughout the embryo, giving rise to a wide variety derivatives including the peripheral nervous system, craniofacial skeleton, pigment cells, and endocrine organs. While much is known about neural crest cells in mammals, birds, amphibians and fish, relatively little is known about their development in non-avian reptiles like snakes and lizards.

Results

In this study, we show for the first time ever trunk neural crest migration in a snake by labeling it with DiI and immunofluorescence. As in birds and mammals, we find that early migrating trunk neural crest cells use both a ventromedial pathway and an inter-somitic pathway in the snake. However, unlike birds and mammals, we also observed large numbers of late migrating neural crest cells utilizing the inter-somitic pathway in snake.

Conclusions

We found that while trunk neural crest migration in snakes is very similar to that of other amniotes, the inter-somitic pathway is used more extensively by late-migrating trunk neural crest cells in snake.