Growth characteristic of hBMSCs. (A) Confluent hBMSCs were trypsinized, fixed and stained with PI. DNA content in cells was examined by flow cytometry. (B) Pre-confluent (density ~80%) and post-confluent (one week after cells reach confluence) hBMSCs from three separate experiments at different cell cycle stages revealed by flow cytometry were quantified. (C) Morphology of hBMSCs at different densities (plated at 5000/cm2 and cultured for one day, one week and five weeks).
Qian et al. BMC Developmental Biology 2010 10:47 doi:10.1186/1471-213X-10-47