Table 1

Expression profiles of investigated candidate genes

Relative expressiona


Candidate gene (abbreviation)

Daphnia pulex gene ID

Embryo

Juvenile


Hox gene

labial (lb)

Dappu-36672

Dappu-97497

1.35

1.598

proboscipedia (pb)

Dappu-44300

Dappu-97500

1.433

1.276

Hox3

Dappu-9456

1.312

1.9

Deformed (Dfd)

Dappu-44270

Dappu-97505

1.35

1.541

Sex combs reduced (Scr)

Dappu-37195

Dappu-44375

Dappu-97506

1.199

1.55

Antennapedia (Antp)

Dappu-44334

Dappu-236216

1.254

1.248

Ultrabithorax (Ubx)

Dappu-9277

Dappu-221891

1.253

1.601

Abdominal-A (Abd-A)

Dappu-29076

Not amplified

Abdominal-B (Abd-B)

Dappu-29045

Dappu-97516

1.235

1.82

Morphogenetic gene

Distal-less (Dll)

Dappu-9287

1.365

1.2

aristaless (al)

Dappu-37455

1.212

1.597

homothorax (hth)

Dappu-4560

Dappu-5009

1.1

1.539

dachshund (dac)

Dappu-94521

Dappu-94522

Dappu-232746

Dappu-232754

1.05

1.591

extradenticle (exd)

Dappu-219790

1.128

2.131

escargot (esg)

Dappu-50534

0.7

1.951

teashirt (tsh)

Dappu-99118

1.276

1.377

epidermal growth factor receptor 1

(EGFR1)

Not predicted

1.193

1.505

epidermal growth factor receptor 2

(EGFR2)

Dappu-9119

Dappu-53919

1.174

1.67

spitz (spi)

Dappu-271340

1.101

1.181

decapentaplegic (dpp)

Dappu-40449

0.992

1.471

wingless (wg)

Dappu-290640

1.306

1.616

hedgehog (hh)

Dappu-290571

1.122

1.579

Endocrine gene

juvenile hormone acid methyltransferase (JHAMT)

Dappu-300180

0.365

2.707

Methoprene-tolerant (Met)

Dappu-247693

0.943

1.584

ultraspiracle (USP)

Dappu-219609

0.775

1.354

ecdysone receptor (EcR)

Dappu-319648

0.736

1.158

insulin-like receptor (InR)

Dappu-270048

0.84

1.956

insulin receptor substrate-1 (IRS-1)

Dappu-52188

Dappu-304473

0.825

1.81

forkhead box O (FOXO)

Dappu-109005

Not amplified

Neuronal gene

tyramine beta-monooxygenase (TBM)

Dappu-1839

1.191

3.897

dopamine beta-monooxygenase (DBM)

Dappu-62540

1.14

1.686


a Expression levels in the presence of the kairomones are displayed relative to control medium, measured by first real-time quantitative PCR. Values in bold indicate more than 1.5-fold increase.

See additional file 1 placing annotations of these genes.

Miyakawa et al. BMC Developmental Biology 2010 10:45   doi:10.1186/1471-213X-10-45

Open Data