Notch112f/tm1Con embryos survive longer than Notch112f/lbd embryos. (A) PCR genotype of an E9.5 litter showed the 280 bp PCR product from Notch1tm1Con allele and the 238 bp product from the Notch112f allele. (B) Yolk sac vascularization of E10.5 Notch112f/tm1Con and Notch1+/12f embryos. (C) Notch112f/tm1Con embryos at E10.5 exhibit heamorrhaging around the heart (arrows). (D) Notch112f//tm1Con and control embryos at E15.5. One Notch112f/tm1Con embryo was defective but the other had no obvious defects. (E) Notch112f/tm1Con and control embryos at E17.5. One Notch112f/tm1Con embryo was defective but the other had no obvious defects. (F) Photo of a litter on postnatal day 1 (P1) which included one pup identified as Notch112f/tm1Con by PCR genotyping below. The pup was indistingishable but died within a few days. (G) PCR genotype of the P1 litter in panel F.
Ge and Stanley BMC Developmental Biology 2010 10:36 doi:10.1186/1471-213X-10-36