Generation of Notch112f/lbd embryos. (A) Diagram of the Notch112f and Notch1lbd alleles. (B) Diagram of mouse Notch1 EGF repeats in Notch112f and Notch1lbd extracellular domains. The EGF repeats with putative O-fucosylation sites are shaded in gray and the mutation in EGF12 is shown. (C) Genotyping by PCR from E9.5 yolk sac DNA of a litter from a Notch112f/12f × Notch1+/lbd cross. Primers 5F and 6R detect the Notch112f and Notch1+ alleles, primers 5F and 9R detect the Notch1lbd allele.
Ge and Stanley BMC Developmental Biology 2010 10:36 doi:10.1186/1471-213X-10-36