Ectopic expression of axolotl BMP-2 and Xenopus laevis Noggin by electroporating the expression plasmids directly into regenerating limbs. Different plasmid combinations were transfected to test the effect of BMP-2 and Noggin on the regeneration process. In order to assess the efficiency of the electroporation in vivo (first column A, D, G), the mRFP expressing plasmid was co-transfected with axolotl BMP-2 (A), Xenopus laevis Noggin (D) or mRFP alone (G; control) in the posterior half of regenerating blastema at the LB stage. The second column (B, E, H) show the resulting phenotypes once the controls (mRFP ectopic expression alone) were fully regenerated and the third column (C, F, I) show their skeletal elements using Victoria blue staining.
Guimond et al. BMC Developmental Biology 2010 10:15 doi:10.1186/1471-213X-10-15