The novel molecular sexing system in Ceratitis capitata. A) Schematic representation of the Y chromosome-derived repetitive sequences. Y114 (red arrows) is the reference sequence, which was used as probe in the in situ experiments and it was the first clone derived from these sequences [14,15]. The AT-rich region (blue boxes) is a 200 bp-long sequence characteristic of Y114. Black arrows indicate CcYf and CcYr primer positions. B) Amplification patterns of genomic DNA from individual males and females using the CcYf/r primers (see Methods). C) Developmental patterns of amplification products from genomic DNA from individual male and female C. capitata. E: embryos 3, 10 and 15 hr after oviposition; L 3rd: 3rd instar larvae; P 7 d: 7 day old pupae; A 3 d: 3 day old adults.
Gabrieli et al. BMC Developmental Biology 2010 10:12 doi:10.1186/1471-213X-10-12