Developmental malformations and hypoplasia caused by ectopic expression of zebrafish ΔNp53 mRNA. (A) Representative images of the head region (upper panel) and quantification (lower panel) of eye size in embryos injected with either zebrafish ΔNp53 mRNA or GFP encoding control mRNA (1 ng/embryo). Eye size was determined by outlining the eye using ImageJ software (NIH) and calculating the eye area within the circumference. Results represent the mean ± SEM of 5 embryos. The asterisk denotes statistically significant difference (t-test, one tailed; p < 0.01). (B) Representative images of embryos and magnification of somites (upper panel) and quantification (lower panel) of somite size. Somite width was determined using ImageJ software (NIH). Results represent the mean of the width of five somites from three individual fish (mean ± SEM; n = 15). Asterices denote statistically significant differences (p < 0.001). The white bar in the GFP mRNA image represents 0.2 mm (10× magnification for all images). In addition to the effects of ΔNp53 mRNA the effects of coinjecting a p21-targeted MO are shown.
Davidson et al. BMC Developmental Biology 2010 10:102 doi:10.1186/1471-213X-10-102