Additional file 2.
Figure S1. miRNA expression detected by RT-PCR. (A) Comparison of relative miRNA expression calculated using a standard curve (as shown in Figure 3A) with the values calculated using ΔCt and efficiency; for each gene the sample with highest expression was normalized to a value of 100. Each point represents the expression of one miRNA in a single sample as determined by both methods, which yielded very similar results (R2 = 0.983). (B) Starting template copy numbers estimated for each miRNA based on threshold cycle (Ct) and amplification efficiency plotted against the values calculated by a linear regression efficiency method (R2 = 0.661).
Format: PDF Size: 143KB Download file
This file can be viewed with: Adobe Acrobat Reader
Arora et al. BMC Developmental Biology 2010 10:1 doi:10.1186/1471-213X-10-1