TLRR associates tightly with the KIFC1 targeting sequence. (A) 200 μg testis lysate (lane 1 and 2) or an aliquot of recombinant HIS6 tagged TLRR (lane 3) were resolved by SDS-PAGE and blotted with TLRR antibody (lane 1 and 3) or preimmune serum (lane 2). (B) Testis lysate was loaded onto a column consisting of KIFC1 targeting peptide-linked resin. Flow through (FT) was collected, the column washed and bound complexes eluted with glycine, pH 2.5 (lane 1) or glycine plus 0.5 M NaCl (lane 2). Coomassie stained gel is shown at top with TLRR western below.
Wang and Sperry BMC Cell Biology 2008 9:9 doi:10.1186/1471-2121-9-9