Table 1

The NDUFAB1 protein selectively binds to the N-terminus of p49 protein (p49-N).

Origin of cDNA Library

Screening bait

Clones positive for NDUFAB1

Main target

Haert

p49-N

95%

NDUFAB1

Hela

p49-N

40%

NDUFAB1

Hela

p49-C

0%

Other

Three separate yeast two-hybrid screenings were performed using p49-N and p49-C as bait, respectively. When p49-N was used to screen human heart cDNA library, 42 out of 44 (95%) of the positive clones that interacted with p49-N were NDUFAB1. When p49-N was used to screen Hela cell cDNA library, 8 out of 20 (40%) of the positive clones were NDUFAB1. However, when p49-C was used to screen Hela cell cDNA library, none of the clones was NDUFAB1. These results indicate that p49-N selectively binds to NDUFAB1.

Zhang et al. BMC Cell Biology 2008 9:8   doi:10.1186/1471-2121-9-8