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The NDUFAB1 protein selectively binds to the N-terminus of p49 protein (p49-N). |
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| Origin of cDNA Library |
Screening bait |
Clones positive for NDUFAB1 |
Main target |
|
|
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| Haert |
p49-N |
95% |
NDUFAB1 |
| Hela |
p49-N |
40% |
NDUFAB1 |
| Hela |
p49-C |
0% |
Other |
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Three separate yeast two-hybrid screenings were performed using p49-N and p49-C as bait, respectively. When p49-N was used to screen human heart cDNA library, 42 out of 44 (95%) of the positive clones that interacted with p49-N were NDUFAB1. When p49-N was used to screen Hela cell cDNA library, 8 out of 20 (40%) of the positive clones were NDUFAB1. However, when p49-C was used to screen Hela cell cDNA library, none of the clones was NDUFAB1. These results indicate that p49-N selectively binds to NDUFAB1. | |||
Zhang et al. BMC Cell Biology 2008 9:8 doi:10.1186/1471-2121-9-8 |
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