Figure 7.

Overexpression of p49/STRAP changes the intracellular NAD/NADH ratio. H9C2 cells were infected with either recombinant adenovirus containing empty vector (control) or p49/STRAP gene (Ad-p49), respectively. After 48 hours, the cells were harvested and the cell lysate samples were subjected to NAD and NADH assay(see methods). The results indicate that p49/STRAP overexpression reduces the intracellular NAD/NADH ratio by approximately 40% in H9C2 cells (p < 0.05, n = 4).