Overexpression of p49/STRAP changes the intracellular NAD/NADH ratio. H9C2 cells were infected with either recombinant adenovirus containing empty vector (control) or p49/STRAP gene (Ad-p49), respectively. After 48 hours, the cells were harvested and the cell lysate samples were subjected to NAD and NADH assay(see methods). The results indicate that p49/STRAP overexpression reduces the intracellular NAD/NADH ratio by approximately 40% in H9C2 cells (p < 0.05, n = 4).
Zhang et al. BMC Cell Biology 2008 9:8 doi:10.1186/1471-2121-9-8