Resolution:
## Figure 5.
Statistical indication that only a minority of the p49 protein is observed in the
cytoplasm. Based on our analyses (see detail in the "Methods" section), approximately less
than 30% of the protein is located in the cytoplasm. Cells were counted using brightfield
microscopy and the images were grouped by days post-transfection as shown in figure
4. It is interesting to note that both the absolute value of, and variability in,
the cytosolic percent seem to be reduced with increased confluence in cell count.
The absolute residuals were regressed against the cell count, and the resulting function
added (+/-) to the function of the original regression, resulting in the "band" shown
in gray. Taken together, the simple least squares regression and residual regression
on cell count account for >43% of the variability in cytosolic percent. This implies
that p49 is localized predominantly in the nucleus. The result is independent of day
of culture, and only slightly influenced by degree of cell confluence.
Zhang |