Statistical indication that only a minority of the p49 protein is observed in the cytoplasm. Based on our analyses (see detail in the "Methods" section), approximately less than 30% of the protein is located in the cytoplasm. Cells were counted using brightfield microscopy and the images were grouped by days post-transfection as shown in figure 4. It is interesting to note that both the absolute value of, and variability in, the cytosolic percent seem to be reduced with increased confluence in cell count. The absolute residuals were regressed against the cell count, and the resulting function added (+/-) to the function of the original regression, resulting in the "band" shown in gray. Taken together, the simple least squares regression and residual regression on cell count account for >43% of the variability in cytosolic percent. This implies that p49 is localized predominantly in the nucleus. The result is independent of day of culture, and only slightly influenced by degree of cell confluence.
Zhang et al. BMC Cell Biology 2008 9:8 doi:10.1186/1471-2121-9-8