NDUFAB1 binds to p49/STRAP. A. Schematic representation indicating that NDUFAB1 binds to the N-terminus of p49/STRAP protein. B. H9C2 cells were transfected with Flag-NDUFAB1 plasmid construct and harvested 24 hours later. An anti-IgG antibody (used as a control) and an anti-p49 antibody were used in the co-immunoprecipitation assay. The results show that p49 antibody co-immunoprecipitated the endogenous p49 protein and the Flag-NDUFAB1 protein, indicating that Flag-NDUFAB1 was bound to endogenous p49 protein. C. Flag-NDUFAB1 expression did not change p49 protein level in the cells (figure 1c).
Zhang et al. BMC Cell Biology 2008 9:8 doi:10.1186/1471-2121-9-8