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Open Access Research article

Identification of a subunit of NADH-dehydrogenase as a p49/STRAP-binding protein

Xiaomin Zhang1*, Gohar Azhar3, Scott Helms1, Ying Zhong1 and Jeanne Y Wei12*

Author Affiliations

1 From the Donald W. Reynolds Department of Geriatrics, The University of Arkansas for Medical Sciences and Geriatric Research, Education, and Clinical Center, Little Rock, AR, USA

2 Central Arkansas Veterans Healthcare System, Geriatric and Extended Care, Little Rock, AR, USA

3 Central Arkansas Veterans Healthcare System, Little Rock, AR, USA

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BMC Cell Biology 2008, 9:8  doi:10.1186/1471-2121-9-8

Published: 29 January 2008

Abstract

Background

The p49/STRAP (or SRFBP1) protein was recently identified in our laboratory as a cofactor of serum response factor that contributes to the regulation of SRF target genes in the heart.

Results

In the present study, we report that NDUFAB1, a nuclear encoded subunit of NADH dehydrogenase, represented the majority of the cDNA clones that interacted with p49/STRAP in multiple screenings using the yeast two-hybrid system. The p49/STRAP and NDUFAB1 proteins interacted and co-localized with each other in the cell. The p49/STRAP protein contains four classic nuclear localization sequence motifs, and it was observed to be present predominantly in the nucleus. Overexpression of p49/STRAP altered the intracellular level of NAD, and reduced the NAD/NADH ratio. Overexpression of p49/STRAP also induced the deacetylation of serum response factor.

Conclusion

These data suggest that p49/STRAP plays a role in the regulation of intracellular processes such as cardiac cellular metabolism, gene expression, and possibly aging.