Comparison of ammonia excretion from the developing amt null strains either expressing or not expressing the respective Amt-RFP fusion protein. Cells from the parental strain (Ax4), the null strains (A-, B-, C-), and the null strains expressing the respective Amt-RFP protein (A-/A24, B-/B35, C-/C54) were plated for development as described in Methods. Excreted ammonia that collected in the buffer-soaked supporting pads for the indicated times post-starvation was measured using an ammonia specific electrode. Error bars give the standard error from three independent experiments.
Kirsten et al. BMC Cell Biology 2008 9:71 doi:10.1186/1471-2121-9-71