Investigation of the cytotoxic effect of cheiradone on BAEC and HDMEC viability. The cytotoxic effect of cheiradone was determined using (A) MTT assay and (B) Active-caspase-3 apoptosis assay. Cells were incubated with cheiradone and staurosporine (1.4 μM) for 24 h. Values significantly different from the control (DMSO) are shown by *. Immunofluorescence photomicrographs for HMDEC were taken as described above and show. (C) control, DMSO (D staurosporine, the arrow indicates a positively stained apoptotic cell and (E) cheiradone.
Hussain et al. BMC Cell Biology 2008 9:7 doi:10.1186/1471-2121-9-7