Additional file 1.
NPCs isolated from E14 embryos retain the capacity to form neurospheres and to differentiate into primary neurons, oligodendrocytes, and astrocytes. NPCs were isolated as described in Methods. (A) Neurospheres were established as previously described  and maintained in N2 medium containing bFGF (10 ng/ml) and epidermal growth factor (EGF, 20 ng/ml). Neurospheres were dissociated and re-formed for two passages (P1 and P2, respectively). Neurospheres were subjected to immunocytochemical analyses using anti-Tuj1, anti-glial fibrillary acidic protein (GFAP), or anti-O4 (oligodendrocyte marker O4) to detect primary neurons, astrocytes, and oligodendrocytes, respectively.
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Jung et al. BMC Cell Biology 2008 9:66 doi:10.1186/1471-2121-9-66