Additional file 8.

Blocking new adhesion formation of spreading mouse embryonic fibroblasts. Lamellipodia but not filopodia are dependent on formation of new adhesions. After 5 minutes of attachment to a glass cover slip, the surface was blocked with denatured BSA and cells were allowed to spread for a further 55 minutes before fixation. Actin was labelled with rhodamine phalloidin and vinculin and Arp2/3 complex with antibodies. Scale bar 10 μm.

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Johnston et al. BMC Cell Biology 2008 9:65   doi:10.1186/1471-2121-9-65