Additional file 5.
Movie of localisation of Arp2/3 complex to filopodia. Arp2/3 complex localises to filopodia of live cells at multiple sites. A GFP tagged p21-arc subunit of Arp2/3 complex and mRFP tagged actin were transiently transfected into MEFs. After spreading on fibronectin for 60 minutes low expressing cells were identified and timelapse sequences taken. Movie 3 is representative of 11 cells in 6 independent experiments. Frames were captured every 10 seconds and movie is displayed at 5 frames per second.
Format: MOV Size: 467KB Download file
Johnston et al. BMC Cell Biology 2008 9:65 doi:10.1186/1471-2121-9-65