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Movie of morphology of mouse embryonic fibroblast spreading on fibronectin. MEFs spread through cycles of filopodia and lamellipodia production making them excellent for the study of both these structures. MEFs in suspension were plated onto fibronectin coated coverslips and a field of view was selected at random. Lines that can be seen on the coverslip are numbered areas for locating cells for correlative fluorescence microscopy. Cells spread identically on marked coverslips compared to unmarked coverslips in all experiments. Frames were captured every 10 seconds. This cell is representative of 30 cells from 4 independent experiments. Movie is displayed at 5 frames per second.

Format: MOV Size: 1.2MB Download file

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Johnston et al. BMC Cell Biology 2008 9:65   doi:10.1186/1471-2121-9-65