I-2 localization by specific antibody staining in human ARPE-19 cells. (A) Extracts from confluent ARPE-19 cells and MCF-7 cells were subjected to Western blotting using affinity-purified sheep antibody specific for human I-2. This antibody only recognized I-2, and its phosphorylated form (minor upper band) in both cell lines. (B) Confluent ARPE-19 cells were fixed, and stained with anti-I-2 antibody as described under Methods. Compressed Z-stack of optical sections from laser confocal microscopy is shown. Scale bar = 20 micron.
Wang and Brautigan BMC Cell Biology 2008 9:62 doi:10.1186/1471-2121-9-62