Adipogenic Differentiation. Results of adipogenic differentiation after 3 weeks. (A) Representative micrographs showing Oil Red O stained lipid inclusions in cultured BMSCs from each experimental group. Magnification bars represent 100 μm in all images. (B) Percentage of cells that had intracellular Oil Red O stained inclusions after 3 weeks. Passage related significant difference in staining was only observed in BMSC cultures from 1-year-old donors (p < 0.05). (C) Percentage of total area that was positively stained with Oil Red O. Passage related significant differences were observed for BMSC cultures from 6-day and 6-week-old donors, and age related differences were only observed between 1-year-old donors and the other groups. The '#' sign indicates a significant difference between passage 1 and passage 6 cultures from similarly aged donors, '‡' indicates a significant difference between the indicated group and any passage 1 groups from younger donors, and '¥' indicates a significant difference between the indicated group and any passage 6 groups from younger donors (p > 0.05). Error bars designate means ± standard deviation (n = 6).
Kretlow et al. BMC Cell Biology 2008 9:60 doi:10.1186/1471-2121-9-60