Analysis of NFκB binding sites in proliferating cells by chromatin immunoprecipitation. Chromatin fragments from proliferating T98G cells were immunoprecipitated with either anti-p50 (A), anti-p52 (B), or anti-RelB (C) antibody and quantified by real-time PCR. Data are presented as the percentage of input and are the means of 2 independent experiments with anti-p50 and anti-p52 or 3 independent experiments with anti-RelB ± S.E. β-globin was used as the negative control. In panel C, (*) represents statistically significant binding compared to β-globin (assessed by t-test).
Terragni et al. BMC Cell Biology 2008 9:6 doi:10.1186/1471-2121-9-6