Analysis of NFκB binding sites in TNFα-stimulated cells by chromatin immunoprecipitation. T98G cells proliferating in serum-containing media were either left untreated or stimulated with TNFα for 15, 30 or 60 minutes. Chromatin fragments were immunoprecipitated with anti-p65 antibody and quantified by real-time PCR. Data are presented as the percentage of input and are the means of 2 independent experiments ± S.E. β-globin was used as the negative control.
Terragni et al. BMC Cell Biology 2008 9:6 doi:10.1186/1471-2121-9-6