Nuclear translocation of FOX01 and FOX03a upon LY294002 treatment. T98G cells were fractionated into nuclear (N; ~2 μg protein) and cytosolic (C; ~25 μg protein) fractions following treatment with LY294002 for the indicated times. Samples were then immunoblotted for the presence of FOX01 and FOX03a. To confirm isolation of nuclear and cytosolic fractions, blots were reprobed with antibodies against PARP (nuclear marker) and 14-3-3 (cytosolic marker). The LY294002 vehicle (DMSO) showed no translocation (data not shown). Shown are representative blots from two independent experiments.
Terragni et al. BMC Cell Biology 2008 9:6 doi:10.1186/1471-2121-9-6