The formation of dispersed aggregates is independent of deacetylase HDAC6, microtubules, and intermediate filament vimentin. (A) The cellular localization of aggregates was determined in N2a and Hela cells transfected with CyPrPEGFP for 24 h in the absence (control) or in the presence of 5 μM tubacin, 5 μM scriptaid, or 5 μM nocodazole. Nuclei were stained with Hoechst (blue). (B) Immunofluorescence analysis of vimentin (red) in N2a and Hela cells transiently transfected with CyPrPEGFP. Nuclei were stained with Hoechst (blue). Red (left panel) and green (middle panel) channels are shown separately, and merged with blue channel (right panel).
Beaudoin et al. BMC Cell Biology 2008 9:59 doi:10.1186/1471-2121-9-59