MDCK apically secreted an endogenous LIF-like factor. MDCK cells were grown on permeable supports for 24 hours and the conditioned media was collected from the apical and basal chambers. The collected media was then analyzed for LIF-like activity using the BA/F3 mLIR-mgp130 cell assay. Results are expressed as the A570 value of cells assayed for proliferation by 3-(4,5-dimethylthiazolyl-2)-2, 5-diphenyl tetrazolium bromide (MTT). Values represent the average activity present in a dilution series of media collected from an individual transwell assayed in triplicate. Two Transwells® are shown with one denoted by a square and one by a triangle. A single representative experiment is shown of n = 3.
Hill and Vernallis BMC Cell Biology 2008 9:53 doi:10.1186/1471-2121-9-53