Caco-2 regulated the direction of secretion of LIF and IL-6 in response to IL-1β. Caco-2 were grown on permeable supports. a, c) The total amount of LIF and IL-6 secreted into both apical and basal chambers was determined by ELISA 24 hours after addition of IL-1β (1 ng/ml) into either the apical (A), basal (B) or both chambers (A+B). b, d) The percentage of LIF and IL-6 secreted into the apical chamber following treatment. Values represent the mean +/- SEM (n = 3). ND (not detected). *p < 0.05 vs. no stimulation; #p < 0.05 apical vs. basolateral stimulation.
Hill and Vernallis BMC Cell Biology 2008 9:53 doi:10.1186/1471-2121-9-53