A, CRP induced a significant increase in chemotaxis in HCAEC. Cell migration was measured using a modified Boyden chamber as described in Methods. The effect of 1–5 μg/ml CRP (non-detoxified and detoxified CRP [CRPdt]) on HCAEC chemotaxis is shown. Endotoxin alone (LPS; 1 ng/ml) had no effect. B, Migration experiments were performed at least twice in triplicate wells. B, rhCRP (1 μg/ml) induced migration as compared with control and rhCRP (1 μg/ml)+antiCRP Ab(1:5). * and ** indicate a statistically significant difference compared with the control cells (p > 0.05 and 0.001 respectively).
Turu et al. BMC Cell Biology 2008 9:47 doi:10.1186/1471-2121-9-47