Co-patching of PLAP and GFP-TMD (Podo) with FAT/CD36. 20 h after transient transfection, Vero cells were incubated for 45 min at 10°C with respective primary antibodies, washed and incubated for 45 min with mixed Cy5 and Cy3 fluorescently-labelled secondary antibodies. PLAP and GFP-TMD were patched with polyclonal rabbit anti PLAP and polyclonal serum KG77 against GFP, respectively. Patching of FAT/CD36 was achieved with monoclonal antibody from Biosource international. FAT/CD36 co-localized with the raft marker protein PLAP and segregated from GFP-TMD. Bar: 10 μm.
Ehehalt et al. BMC Cell Biology 2008 9:45 doi:10.1186/1471-2121-9-45