Table 1

Effect of various N-WASP constructs onto cell ploidy.

Vector

Total cell number

Number of binucleated cells

% of binucleated cells


GFP

419

17

4.06

GFP VCA

527

61

11.57

GFP CA

629

30

4.77

GFP N-WASP

351

19

5.41

GFP N-WASP ΔA

130

7

3.38


NIH3T3 cells were transfected with indicated constructs. Three days after transfection cells were harvested and a fifth seeded onto glass coverslips. Two hours after seeding cells were fixed. Nuclei number of transfected cells was determined after F-actin and DNA staining respectively with TRITC-coupled phalloidin and DAPI. Total cell number and the number of binucleated cells from a representative experiment are presented as well as the deduced percentage of binucleated cells.

Bompard et al. BMC Cell Biology 2008 9:42   doi:10.1186/1471-2121-9-42

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