The recruitment of AR and β-Catenin to Wnt signaling target genes as well as PSA promoter and enhancer region using CHIP analysis. LNCaP cells in phenol-red free RPMI with 5% charcoal-stripped serum were treated with either 10 nM DHT, 100 ng/ml Wnt3A, or no treatment for 16 hours before cross-linking. Anti-β-Catenin (A, B, G and H) and anti-AR (C, D, E and F) antibodies, together with negative control IgG were then used for immunoprecipitation. After reverse crosslinking and DNA purification, PCR products were analyzed using the Agilent 2100 bioanalyzer. Percent of input is shown here to compare the levels of β-Catenin or AR at the promoter or enhancer region of PSA, or the promoter region of Cyclin D1 or Myc.
Schweizer et al. BMC Cell Biology 2008 9:4 doi:10.1186/1471-2121-9-4