The putative NLS of PARP-2 contains several conserved lysine residues. A) Three lysines in the putative NLS of PARP-2 are conserved between different mammalian species. Sequences were obtained from NCBI and alignments were performed using ClustalW2. B) Schematic illustration of PARP-2 K → R mutant proteins used in this study: K19, K20, K36 and K37 were changed to arginine using site-directed mutagenesis. Double and quadruple mutants were generated. C) HA-tagged wild type (wt) PARP-2 or the indicated double or quadruple mutants were expressed in HEK293T cells and expression was analyzed by western blot using a monoclonal anti-HA antibody. 100 μg of whole cell extracts were used, endogenous PARP-1 levels served as loading control.
Haenni et al. BMC Cell Biology 2008 9:39 doi:10.1186/1471-2121-9-39